Developing of Dna-marker to the Fusarium Oxysporum F. Sp. Lycopersici Resistance Genes of Tomato

Fusarium wilt caused by Fusarium oxysporum f. sp. lycopersici is a destructive disease of tomato crops worldwide. The use of resistant varieties is the best strategy for disease control. In the present study we analyze eight tomato lines and hybrids for Fusarium wilt disease resistance by polymerase chain reaction. Total genomic DNA was extracted from young leaves of three-week-old plants of tomato. Results of PCR of eight tomato lines and hybrids indicated that there are one dominant heterozygote, two recessive homozygotes and five dominant homozygotes. Also, results of polymerase chain reaction showed that it needs less time and is cheaper. Also by using this method, it is possible to determine genotype of plant (homozygote or heterozygote) without presence of the pathogen. Therefore, PCR technique was used in the identification gene I2 conferring resistance to F. oxysporum f. sp. lycopersici.